Effect of Topical Application of Black Seed Oil (Nigella Sativa) on Wound Healing in Diabetic Rat Ulcer Model

Eliza Arman; Almahdy A; Putri Dafiriani; Dedy Almasdy

doi:10.25077/mka.v47.i4.p447-456.2024

Authors

Eliza Arman Faculty of Farmacy, Andalas University, Padang, Indonesia/ Department of Medical Laboratory Technology, STIKES Syedza Saintika, Padang, Indonesia
Almahdy A Faculty of Farmacy, Andalas University, Padang, Indonesia
Putri Dafiriani Department of Biomedical, STIKES Syedza Saintika, Padang, Indonesia
Dedy Almasdy Faculty of Farmacy, Andalas University, Padang, Indonesia

DOI:

https://doi.org/10.25077/mka.v47.i4.p447-456.2024

Keywords:

Black seed oil, Wouand healing, TNF alpha, Histophatology

Abstract

Objective : This study aims to evaluate the use of topical black seed oil on wound healing in a diabetic rat model. Method:10 experimental rats were induced with streptozotocin at a dose of 55 mg/kg BW. Diabetic rats were anesthetized and then the wound was made with a biopsy push with a diameter of 10 mm. The rats were divided into 2 groups, namely the untreated group and the topical treatment group with 1 ml of 100% Black Seed Oil every day for 14 days. Wound healing evaluation was carried out on days 7 and 14. Gene expression analysis was performed using molecular technique RT PCR an histopathologically using He staining. Data analysis used T test. Result: On day 7 there was a decrease in TNF expression with p <0.05, while on day 14 it also showed a decrease in TNF alpha expression with p <0.01. Histopathological showed positive development by showing better wound healing with complete epithelialization, granulation with lower inflammatory cells, higher fibroblasts and collagen compared to the control group. Conclusion :Black seed oil can increase the speed of wound healing in diabetic rat models by inhibiting TNF alpha expression.

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