Model Hewan Coba Diabetes Mellitus Yang Diinduksi Streptozocin Terhadap Kadar Gula Darah Dan Pankreas
Abstract
Objective : The purpose of this study was to determine blood sugar levels and histopathological features of the pancreas in Wistar rats (Rattus norvegicus) induced by Streptozotocin. Methods: The type of research conducted is experimental laboratory in vivo. The research design chosen was True Experimental Pre Test and Post Test Control Group Design with 24 test animals and divided into six different treatment groups. The results were analyzed using SPSS software, and for the histopathological description the rats were dissected and the pancreas was taken for preparation and then stained using the Hematoxylin Eosin (HE) method. Results: The results of this study indicate that the histopathological description of pancreatic tissue is damaged which can be seen from the occurrence of cell necrosis, cell degeneration, collections of inflammatory cell , atherosclerosis and vascular congestion are found. Blood sugar levels of rats given a combination of STZ and high-fat diet significantly increased blood sugar levels (P value < 0.05). Conclusion : There is a significant relationship between blood sugar levels and pancreatic histopathology in Wistar rats (Rattus norvegicus) induced by Streptozotocin and high-fat diet.
Keywords: Diabetes mellitus; Blood Sugar Levels; Streptozotocin; Pancreas Histology
Keywords
Full Text:
PDFReferences
Soelistijo SA, Lindarto D, Decroli E, Permana H, Sucipto KW, Kusnadi Y, et al. Pedoman pengelolaan dan pencegahan diabetes melitus tipe 2 dewasa di Indonesia 2019. Perkumpulan Endokrinol Indones. 2019;1–117.
Azis WA, Muriman LY, Burhan SR. Hubungan Tingkat Pengetahuan dengan Gaya Hidup Penderita Diabetes Mellitus. J Penelit Perawat Prof. 2020;2(1):105–14.
Lubis MA, Farmasi M, Farmasi D. Analisis Cost-Effectivenees Penggunaan Antidiabetik Oral Pada Pasien Diabetes Melitus Tipe 2 Rawat Jalan Peserta Bpjs Di Rsu Haji Medan. 2018;2(3):128–47.
Husna F, Suyatna FD, Arozal W, Purwaningsih EH. Model Hewan Coba pada Penelitian Diabetes. Pharm Sci Res. 2019;6(3):131–41.
Saputra NT, Suartha IN, Dharmayudha AAGO. Agen Diabetagonik Streptozotocin untuk Membuat Tikus Putih Jantan Diabetes Mellitus. Bul Vet Udayana. 2018;10(2):116.
Zulkarnain. Perubahan kadar glukosa darah puasa pada tikus. J Kedokt Univ SYIAH KUALA. 2013;13:71–6.
Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: An overview K. Indian J Med Res. 2012;136(1):451–72.
Erwin et al. EKSPRESI INSULIN PADA PANKREAS MENCIT (Mus musculus) YANG DIINDUKSI DENGAN STREPTOZOTOCIN BERULANG. 2013;7:97–100.
Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216–26.
Ragbetli C, Ceylan E. Effect of streptozotocin on biochemical parameters in rats. Asian J Chem. 2010;22(3):2375–8.
Mehta BK, Banerjee S. Characterization of Cognitive Impairment in Type 2 Diabetic Rats. Indian J Pharm Sci. 2017;79(5):785–93.
Guo X xuan, Wang Y, Wang K, Ji B ping ZF. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection. J Zhejiang Univ Sci B. 2018;19(7):559–69.
Vatandoust N, Rami F, Salehi A, Khosravi S, Dashti G, Eslami G et al. Novel High-Fat Diet Formulation and Streptozotocin Treatment for Induction of Prediabetes and Type 2 Diabetes in Rats. Adv Biomed Res. 2018;7(1):107.
Girault FM, Sonnay S, Gruetter R DJ. Alterations of Brain Energy Metabolism in Type 2 Diabetic Goto-Kakizaki Rats Measured In Vivo by 13C Magnetic Resonance Spectroscopy. Neurotox Res. 2019;36(2):268–78.
Calvo-Ochoa E, Hernández-Ortega K, Ferrera P, Morimoto S AC. Short-term high-fat-and-fructose feeding produces insulin signaling alterations accompanied by neurite and synaptic reduction and astroglial activation in the rat hippocampus. J Cereb Blood Flow Metab. 2014;34(6):1001–8.
Hussain Y, Jain SK SP. Short-term westernized (HFFD) diet fed in adolescent rats: Effect on glucose homeostasis, hippocampal insulin signaling, apoptosis and related cognitive and recognition memory function. Behav Brain Res. 2019;361:113–21.
Li RJ, Qiu SD, Tian H ZS. Diabetes induced by multiple low doses of STZ can be spontaneously recovered in adult mice. Dongwuxue Yanjiu. 2013;34(3):238–43.
Firdaus, Marliyati SA RK. Model Tikus Diabetes Yang DIiinduksi Sterptozotocin- Sukrosa Untuk Pendekatan Penelitian Diabetes Streptozotocin , Sucrose- Induce Diabetic Male Rats Model for Research. J MKMI. 2016;12(1):29–34.
