Eksplorasi bakteri gram negatif endofit tanaman kunyit (Curcuma longa L.) yang memiliki kemampuan Quorum Quenching

Meika Meidina Yuanita, Eka Ardiani Putri, Mahyarudin Mahyarudin✉, Mardhia Mardhia, Ambar Rialita
DOI: https://doi.org/10.25077/mka.v42.i2.p80-90.2019

Abstract

Tujuan: Penelitian ini bertujuan untuk mengetahui karakter bakteri Gram negatif endofit tanaman kunyit yang memiliki kemampuan Quorum Quenching (QQ). Metode: Bakteri Gram negatif endofit tanaman kunyit diisolasi, dimurnikan, dan disubkultur pada media NA dengan metode cawan gores. Uji QQ dilakukan dengan metode disc diffusion untuk mengukur pembentukan zona hambat pada bakteri uji Chromobacterium violaceum. Bakteri yang memiliki kemampuan QQ dikarakterisasi berdasarkan morfologi koloni, morfologi sel dan aktivitas biokimia. Hasil: Sebanyak 21 isolat bakteri endofit berhasil diisolasi dan dimurnikan dari tanaman kunyit dan 14 isolat diantaranya merupakan bakteri Gram negatif. Semua isolat bakteri Gram negatif endofit memiliki kemampuan QQ yang berkisar antara 6,5-13,5 mm. Satu isolat yang memiliki kemampuan QQ paling besar adalah isolat 6 dengan hasil identifikasi termasuk ke dalam genus Pseudomonas. Simpulan:Bakteri Gram negatif endofit tanaman kunyit yang memiliki kemampuan QQ paling potensial adalah genus Pseudomonas.

 

Keywords

bakteri gram negatif endofit; Curcuma longa L.; quorum quenching; Chromobacterium violaceum

Full Text:

PDF

References

Tortora GJ, Funke BR, Case CL. Microbiology: An introduction. 10th ed. California: Benjamin & Cummings; 2010.

Kabir AH, Roy AG, Alam MF, Islam R. Detection of quorum sensing signals in Gram-negative bacteria by using reporter strain CV026. Not Sci Biol. 2010; 2(4):72-5. doi: 10.15835/nsb244863.

Yang L, Liu Y, Sternberg C, Molin S. Evaluation of enoyl-acyl carrier protein reductase inhibitors as Pseudomonas aeruginosa quorum-quenching reagents. Molecules. 2010; 15(2):780-92. doi: 10.3390/molecules15020780.

Deep A, Chaudhary U, Gupta V. Quorum sensing and bacterial pathogenicity: From molecules to disease. J Lab Physicians. 2011; 3(1):4-11. doi: 10.4103/0974-2727.78553.

May AL, Eisenhauer ME, Coulston KS, Campagna SR. Detection and quantitation of bacterial acylhomoserine lactone quorum sensing molecules via liquid chromatography-isotope dilution tandem mass spectrometry. Anal Chem. 2012; 84(3):1243-52. doi: 10.1021/ac202636d.

Krishnan T, Yin WF, Chan KG. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa PAO1 by ayurveda spice clove (Syzygium aromaticum) bud extract. Sensors. 2012; 12(4):4016-30. doi: 10.3390/s120404016.

Rukayadi Y, Hwang JK. Pencegahan quorum sensing: Suatu pendekatan baru dalam mengatasi infeksi bakteri. Medicinus. 2009; 22(1):22-7.

Kalia, Chandra V. Quorum sensing inhibitors: An overview. Biotechnol Adv. 2013; 31(2):224-45. doi: 10.1016/j.biotechadv.2012.10.004.

Yang CH, Li YH. Chromobacterium violaceum infection: A clinical review of an important but neglected infection. J Chin Med Assoc. 2011; 74(10):435-41. doi: 10.1016/j.jcma.2011.08.013.

Skogman ME, Kanerva S, Manner S, Vuorela PM, Fallarero A. Flavones as quorum sensing inhibitor identified a newly optimized screening platform using Chromobacterium violaceum as reporter bacteria. Molecules. 2016; 21(9):E1211. doi: 10.3390/molecules21091211.

Tiwit W, Bustanussasalam B, Harmastini S, Partomuan S. Isolasi dan identifikasi kapang endofit dari tanaman kunyit (Curcuma longa L.) sebagai penghasil antioksidan. Biopropal Industri. 2016; 7(1):9-16.

Bacha K, Tariku Y, Gebreyesus F, Zerihun S, Mohammed A, Weiland-Bräuer N, Mulat M. Antimicrobial and anti-quorum sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiol. 2016; 16(1):139. doi: 10.1186/s12866-016-0765-9.

Nazzaro F, Fratianni F, Coppola R. Quorum sensing and phytochemicals. Intl J Mol Sci. 2013; 14(6):12607-19. doi: 10.3390/ijms140612607.

Gottardi D, Bukvicki D, Prasad S, Tyagi AK. Beneficial effects of spices in food preservation and safety. Front Microbiol. 2016; 7:1394. doi: 10.3389/fmicb.2016.01394.

Maehara S, Simanjuntak P, Kitamura C, Ohashi K, Shibuya H. Cinchona alkaloids are also produced by an endophytic filamentous fungus living in cinchona plant. Chem Pharm Bull. 2011; 59(8):1073-4. doi: 10.1248/cpb.59.1073.

Haryono. Ayo mengenal tanaman obat. Jakarta: Badan Penelitian dan Pengembangan Pertanian Kementrian Pertanian; 2012.

Sulistiyani TR. Keragaman Bakteri Endofit Tanaman Kunyit Putih (Curcuma zeodaria) dan Toksisitasnya terhadap Embrio Ikan Zebra. Bogor: Institut Pertanian Bogor; 2014.

Fitriyah D. Karakterisasi Bakteri Penghasil Asil Homoseril Lakton Laktonase dan Asilase yang diisolasi dari Lahan Pertanian [Tesis]. Bogor: Institut Pertanian Bogor; 2015.

Holt JG, NR Krieg. Bergey’s manual of determinative bacteriology. 9th ed. Baltimore: Williams & Wilkins; 2000. p.187.

Agusta A. Biologi dan kimia jamur endofit. Bandung: ITB; 2009. p.115-8.

Munif A, Hipi A. Potensi bakteri endofit dan rhizosfer dalam meningkatkan pertumbuhan jagung. Prosiding Seminar National Serelia. Bogor: Institut Pertanian Bogor; 2012.

Dwidjoseputro. Dasar-dasar mikrobiologi. Jakarta: Djambatan; 2010.

Charlena, Haris A, Karwati. Degradasi Hidrokarbon pada Tanah Tercemar Minyak Bumi dengan Isolat A10 dan D8. Prosiding Seminar Nasional Sains II. Bogor: Institut Pertanian Bogor; 2009.

Mas’ud F. Media, Isolasi, Sterilisasi, Peremajaan, dan Penyimpanan Mikroba; 2013. Diunduh dari: https://www.scribd.com/presentation/100436268/Media-Isolasi-Sterilisasi-Peremajaan-Dan

Vasconcelos ATR, de Almeida DF, Hungria M, Guimarães CT, Antônio RV, Almeida FC, et al. The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability. Proc Nat Aca Scien of U S A. 2003; 100(20): 11660–11665. doi: 10.1073/pnas.1832124100.

Pratiwi ST. Mikrobiologi farmasi. Jakarta: Erlangga; 2009.

Fitriyah D, Jose C, Saryono. Skrining aktivitas antimikroba dan uji fitokimia dari kapang endofitik tanaman dahlia (Dahlia variabilis). J Ind Che Acta. 2009; 3(2):50-5.

Ardani YB. Uji aktivitas antibakteri dan anti-quorum sensing fraksi dari ekstrak etanol daun salam (Eugenia polyantha Wight). [Skripsi]. Surabaya: Fakultas Farmasi, Universitas Katolik Widya Mandala; 2013.

Packiavathy IA, Priya S, Pandian SK, Ravi AV. Inhibition of biofilm development of uropathogens by curcumin-an anti-quorum sensing agent from Curcuma longa. Food Chem. 2014; 148:453-60. doi: 10.1016/j.foodchem.2012.08.002.

McClean KH, Winson MK, Fish L, Taylor A, Chhabra SR, Camara M, et al. Quorum sensing and Chromobacterium violaceum: Exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones. Microbiology. 1997; 143(Pt 12):3703-11. doi: 10.1099/00221287-143-12-3703.

Morohoshi T, Kato M, Fukamachi K, Kato N, Ikeda T. N-Acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472. FEMS Microbiol Lett. 2008; 279(1):124-30. doi: 10.1111/j.1574-6968.2007.01016.x.

Sulistiyani TR, Lisdiyanti P, Lestari Y. Population and diversity of endophytic bacteria associated with medicinal plant Curcuma zedoaria. Microbiology Indonesia. 2014; 8(2):64.

Miller KI, Qing C, Sze DM, Roufogalis BD, Neilan BA. Culturable endophytes of medicinal plants and the genetic basis for their bioactivity. Microb Ecol. 2012; 64(2):431-49. doi: 10.1007/s00248-012-0044-8.

Fitriani A, Ayuningtyas DP, Kusnadi. Inhibition of quorum sensing in Chromobacterium violaceum CV026 by violacein produced by Pseudomonas aeruginosa. Journal of Experimental Biology and Agricultural Sciences. 2016; 4(1):103-8. doi: 10.18006/2016.4(1).103.108.

Huang JJ, Han JI, Zhang LH, Leadbetter JR. Utilization of acylhomoserine lactone quorum signals for growth by a soil pseudomonad and Pseudomonas aeruginosa PAO1. Appl Environ Microbiol. 2003; 69(10):5941-9. doi: 10.1128/aem.69.10.5941-5949.2003.

Uroz S, Dessaux Y, Oger P. Quorum sensing and quorum quenching: The yin and yang of bacterial communication. Chembiochem. 2009; 10(2):205-16. doi: 10.1002/cbic.200800521.

Lin YH, Xu JL, Hu J, Wang LH, Ong SL, Leadbetter JR, Zhang LH. Acylhomoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes. Mol Microbiol. 2003; 47(3):849-60. doi: 10.1046/j.1365-2958.2003.03351.x.

Leadbetter JR, Greenberg EP. Metabolism of acylhomoserine lactone quorum-sensing signals by Variovorax paradoxus. J Bacteriol. 2000; 182(24):6921-6. doi: 10.1128/JB.182.24.6921-6926.2000.

Utari PD, Vogel J, Quax WJ. Deciphering physiological functions of AHL quorum quenching acylases. Front Microbiol. 2017; 8:1123. doi: 10.3389/fmicb.2017.01123.



Bookmark and Share
-->