Aktivitas Antijamur Infusa Daun Dandang Gendis (Clinacanthus nutans (Burm.f.) Lindau) Terhadap Pertumbuhan Candida albicans

Kenny Jhody S✉
DOI: https://doi.org/10.25077/mka.v46.i4.p650-661.2023

Abstract

Tujuan: Mengetahui aktivitas antijamur infusa daun dandang gendis terhadap pertumbuhan Candida albicans dan mengetahui konsentrasi efektif infusa daun dandang gendis pada pertumbuhan Candida albicans; Metode: Skrining fitokimia infusa daun dandang gendis dilakukan dengan pengujian secara kualitatif. Pembuatan infusa daun dandang gendis dilakukan dengan merebus daun dandang gendis selama 15 menit dalam akuades yang telah dipanaskan dengan suhu 90oC. Pengujian aktivitas antijamur menggunakan metode difusi cakram dengan konsentrasi 20%, 40%, 60%, 80%, dan 100%. Flukonazol 25 ug/cakram dan Itrakonazol 8ug/cakram digunakan sebagai kontrol positif dan akuades steril digunakan sebagai kontrol negatif; Hasil: Berdasarkan hasil uji metabolit, didapatkan kandungan metabolit sekunder infusa daun dandang gendis yaitu fenol, saponin, tanin, terpenoid, alkaloid, dan flavonoid. Metabolit sekunder dominan pada infusa daun dandang gendis adalah fenol (+++). Pengujian infusa daun dandang gendis tidak menunjukkan adanya zona hambat meskipun diujikan pada konsentrasi 100%; Kesimpulan: Infusa daun dandang gendis tidak memiliki aktivitas antijamur terhadap Candida albicans.

Keywords

Antijamur; Clinacanthus nutans; Candida albicans

Full Text:

PDF

References

Spampinato C, Leonardi D. Candida infections, causes, targets, and resistance mechanisms: traditional and alternative antifungal agents. BioMed Research International. 2013. p. 1–13.

Tsui C, Kong EF, Jabra R, Mary A. Pathogenesis of candida albicans biofilm. Vol. 74, Pathogens and disease. 2016.

Jiwintarum Y, Urip, Wijaya AF, Diarti MW. Media alami untuk pertumbuhan jamur candida albicans penyebab kandidiasis dari tepung biji kluwih (artocarpus communis). J Kesehat Prima. 2017;11(2):158–70.

NCHS and CDC. Invasive candidiasis risk & prevention [Internet]. [cited 2020 Jul 21]. Available from: https://www.cdc.gov/fungal/diseases/candidiasis/index.html

Kementerian Kesehatan Republik Indonesia 2016. Profil Kesehatan Indonesia Tahun 2015. Health Statistics. 2016.

Kementerian Kesehatan Republik Indonesia 2017. Profil Kesehatan Indonesia Tahun 2016. Health Statistics. 2017.

Pappas PG, Kauffman CA, Sobel J De. Clinical practice guideline for the management of candidiasis: 2016 update by the infectious diseases society of america. Clin Infect Dis. 2016;62(4):e1–50.

Astuti N. Perbandingan resistensi candida albicans dan candida non albicans terhadap flukonazol dan nistatin (kajian pada bilasan orofaring penderita human immunodeficiency virus di rsup dr. sardjito, yogyakarta). Gadjah Mada; 2013.

Sharma P, More S, Raut S, Rathod V. In vitro antifungal susceptibility pattern of oropharyngeal and oesophageal candida spesies in hiv infected patients. Internaional J Heal Sci Res. 2013;3(5):1–6.

Yang HS, Peng TW, Madhavan P, Abdul Shukkoor M, Akowuah G. Phytochemical analysis and antibacterial activity of methanolic extract of Clinacanthus nutans leaf. Int J Drug Dev Res. 2013;5(3):349–55.

Nurulita Y, Dhanutirto H, Soemardji AA. Penapisan aktivitas dan senyawa antidiabetes ekstrak air daun dandang gendis (clinacanthus nutans). J Natur Indones. 2008;1:1.

Alam A, Ferdosh S, Ghafoor K, Hakim A, Juraimi AS, Khatib A, et al. Clinacanthus nutans: a review of the medicinal uses, pharmacology and phytochemistry. Asian Pac J Trop Med. 2016;9(4):402–9.

Kong HS, Abdullah Sani N. Antimicrobial properties of the acetone leaves and stems extracts of clinacanthus nutans from three different samples/areas against pathogenic microorganisms. Int Food Res J. 2018;25(4):1698–702.

Zhang QW, Lin L-G, Ye W-C. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20.

Sharma K, Ko EY, Assefa A De, Ha S, Nile SH, Lee ET, et al. Temperature-dependent studies on the total phenolics, flavonoids, antioxidant activities, and sugar content in six onion varieties. J Food Drug Anal. 2015;23(2):243–52.

Kementerian Kesehatan. Formularium Ramuan Obat Tradisional Indonesia. 2017. 8 p.

Rusita YD. Flavonoids content in extracts secang (caesalpina sappani.) maceration method infudation analysis and visible ultraviolet specttrophotometer. Int J Med. 2016;5(4):176–81.

Widaningrum. Uji potensi anti fungi infusa daun sirih merah (piper crocatum ruiz&pav) terhadap candida albicans atcc 10231 secara in vitro. Univ Sanata Dharma. 2008;1:2.

Federer W. Experimental design theory and application. New Delhi, India: Oxford & IBH; 1967.

Nadifah F, Fatimah S, Lamablawa IYB. Pengaruh infusa daun sambiloto (andrographis paniculata nees) terhadap pertumbuhan bakteri shigella dysenteriae secara in vitro. J Heal. 2014;1(1):29.

Fardyansjah H, Aziz SA, Melati M. Perbedaan waktu panen daun terhadap produksi dan kadar flavonoid tempuyung (sonchus arvensis l.). J Hort Indones. 2017;8(2).

Khoo LW, Audrey Kow S, Lee MT, Tan CP, Shaari K, Tham CL, et al. A comprehensive review on phytochemistry and pharmacological activities of clinacanthus nutans (burm.f.) lindau. Vol. 2018, Evidence-based Complementary and Alternative Medicine. Hindawi Limited; 2018.

Tyasrini E, Susantina W. Hubungan antara sifat dan metabolit candida spp. Jkm. 2006;6:52–67.

Amir, Sari NI, Darmawati, Dewi SS. Tepung talas sebagai media alternatif pertumbuhan candida albicans dan aspergillus sp. Pros Semin Nas Mhs Unimus. 2018;1:78–85.

Aslani N, Shokohi T, Ataollahi MR, Ansari S, Gholampour Y, Jeihooni AK, et al. In vitro activity of four triazole antifungal drugs against clinically common and uncommon yeast species. Curr Med Mycol [Internet]. 2019;5(4):14–9. Available from: /pmc/articles/PMC7034789/

Berkow EL, Lockhart SR. Fluconazole resistance in candida species: a current perspective. Infect Drug Resist. 2017 Jul 31;10:237–45.

Vandeputte P, Ferrari S, Coste AT. Antifungal resistance and new strategies to control fungal infections. Int J Microbiol. 2012;2012.

De OSG, Vasconcelos C, Lopes A. Candida infections and therapeutic strategies: mechanisms of action for traditional and alternative agents. Front Microbiol. 2018;9.

Hasim S, Coleman JJ. Targeting the fungal cell wall: current therapies and implications for development of alternative antifungal agents. Futur Med Chem. 2019;11(8):869–83.

Gao J, Wang H, Li Z, Wong AHH, Wang YZ, Guo Y, et al. Candida albicans gains azole resistance by altering sphingolipid composition. Nat Commun. 2018;9(1).

Bayot ML, Bragg BN. Antimicrobial susceptibility testing. In: Treasure Island (FL) [Internet]. StatPearls Publishing; 2020. Available from: https://www.ncbi.nlm.nih.gov/books/NBK539714/

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017 Dec 1;6(4):42.

Li J, Xie S, Ahmed S, Wang F, Gu Y, Zhang C, et al. Antimicrobial activity and resistance: influencing factors. Front Pharmacol. 2017;8:364.

WHO. Laboratory manual for diagnosis of fungal opportunistic infections in HIV/AIDS patients. World Health Organization. 2009. 86–90 p.

Wang L, Fan D, Chen W, Terentjev EM. Bacterial growth, detachment and cell size control on polyethylene terephthalate surfaces. Sci Rep. 2015 Oct 14;5.

Ensamory M, Rahmawati, Rousdy D. Aktivitas antijamur infusa kulit buah jeruk siam(citrus nobilis) terhadap aspergilus niger emp1 u2. Laboran Med. 2017;1(2).

National Center for Biotechnology Information. PubChem Compound Summary for CID 962, Water. Natl Libr Med [Internet]. 2021; Available from: https://pubchem.ncbi.nlm.nih.gov/compound/Water

Saidi N, Ginting Bi, Murniana, Mustanir. Analisis metabolit sekunder. 1st ed. Banda Aceh: Syiah Kuala University Press; 2018. 23–24 p.

Septiana A, Asnani A. Kajian sifat fisikokimia ekstrak rumput laut coklat sargassum duplicatum menggunakan berbagai pelarut dan metode ekstraksi. J Agrointek. 2012;6(1).

Arullappan S, Rajamanickam P, Thevar N, Kodimani CC. In vitro screening of cytotoxic, antimicrobial and antioxidant activities of clinacanthus nutans (acanthaceae) leaf extracts. Trop J Pharm Res. 2014;13(9):1455–61.

Laurence YM, Sanagi MM, Khan MS, Majid MHA, Sahjadi MS. Effect of drying methods on the colour parameter, rehydration capacity and antioxidant activity of clinacanthus nutans leaves. Malaysian J Chem. 2019;21(1).

Sulaksono FB, Syamsudin AB. Koreksi kadar flavonoid dan toksisitas dalam ekstrak tempuyung (sonchus arvensis) dan pegagan (centella asiatica). J Konversi. 2012;1(2).

Gupita CN, Rahayuni A. Pengaruh berbagai ph sari buah dan suhu pasteurisasi terhadap aktivitas antioksidan dan tingkat penerimaan sari kulit buah manggis. J Nutr Coll. 2012;1(1).

Fong Y, Piva T, Dekiwadia C, Urban S, Huynh T. Comparison of cytotoxicity between extracts of clinacanthus nutans (burm. f.) lindau leaves from different locations and the induction of apoptosis by the crude methanol leaf extract in D24 human melanoma. BMC Complement Altern Med. 2016;368.

Sari KIP, Periadnadi, Nasir N. Uji antimikroba ekstrak segar jahe-jahean (zingiberaceae) terhadap staphylococcus aureus, escherichia coli dan candida albicans. J Biol Univ Andalas. 2013;2(1):20–4.

Costa-de-oliveira S, Rodrigues AG. Candida albicans antifungal resistance and tolerance in bloodstream infections: the triad yeast-host-antifungal. Microorganisms. 2020;8(2):154.

Rubio RG, Oliveira HC de, Rivera J, Contador NT. The fungal cell wall: candida, cryptococcus, and aspergillus species. Front Microbiol. 2020;10(2993).



Bookmark and Share
-->