Efek Protektif Madu Hutan (Apis dorsata) Kalimantan Barat terhadap Gambaran Histopatologi Ginjal Tikus Galur Wistar yang diberi Pajanan Aspirin

Riyda Leovany✉
DOI: https://doi.org/10.25077/mka.v46.i2.p367-376.2023

Abstract

Tujuan: untuk mengetahui efek protektif madu hutan (Apis dorsata) terhadap kerusakan ginjal tikus galur Wistar yang diberi pajanan aspirin. Metode: Penelitian ini menggunakan sampel bahan biologi tersimpan berupa blok-blok parafin ginjal tikus galur Wistar yang dibagi ke dalam 4 kelompok yaitu kelompok kontrol normal, kelompok kontrol negatif yang diberi aspirin 400 mg/kgBB, kelompok perlakuan 1 dan kelompok perlakuan 2 yang diberikan madu hutan secara oral dengan dosis masing-masing 1,55 ml/kgBB dan 3,1 ml/kgBB kemudian aspirin masing-masing 400 mg/kgBB pada satu jam berikutnya. Perlakuan diberikan selama 7 hari. Hasil: Madu Hutan dengan dosis 3,1 ml/kgBB memiliki perbedaan kerusakan glomerulus yang bermakna terhadap kelompok control negatif dan tidak memiliki perbedaan tubulus proksimal normal yang bermakna terhadap kelompok kontrol normal. Kesimpulan: Madu hutan (Apis dorsata) memiliki efek protektif terhadap gambaran histopatologi ginjal tikus galur Wistar yang diberi pajanan aspirin.

Keywords

Forest honey; renal histopathology; aspirin

Full Text:

PDF

References

Kumar V, Abbas AK, Aster JC. Robbins basic pathology. 10th ed. Philadelphia, PA: Elsevier - Health Sciences Division; 2017.

Burkhart R, Shah N, Lewin M. Sildenafil induced acute interstitial nephritis. Case Reports in Nephrology. 2015;1-3.

McAninch J, Lue T, Smith D. Smith & Tanagho's general urology. 18th ed. New York: McGraw-Hill Professional; 2013.

Chowdry AM, Azad H, Mir I, Najar MS, Ashraf BM, Muzafar WM, Ahmed WI. Drug-induced acute interstitial nephritis: Prospective randomized trial comparing oral steroids and high-dose intravenous pulse steroid therapy in guiding the treatment of this condition. Saudi J Kidney Dis Transpl. 2018;29(3):598-607.

Rahmadanita FF, Sumarno. Kajian pustaka efek samping aspirin : Aspirin-Exacerbated Respiratory Disease (AERD). Pharmaceutical Journal of Indonesia. 2020;5(1).

Schrör K. Acetylsalicylic acid. 2nd ed. Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA; 2016.

Bouzenna H, Dhibi S, Samout N, Rjeibi I, Talarmin H, Elfeki A, Hfaiedh N. The protective effect of Citrus limon essential oil on hepatotoxicity and nephrotoxicity induced by aspirin in rats. Biomed Pharmacother. 2016 Oct;83:1327-1334.

Asif S, Mudassir S, Toor RS. Histological effects of Nigella sativa on aspirin-induced nephrotoxicity in albino rats. J Coll Physicians Surg Pak. 2018;28(10):735-738

Roosdiana A, Permata FS, Graf PS. The sumbawa forest honey as preventive agent for organ damaged caused by environmental pollution. IOP Conf Ser: Earth Environ Sci. 2019;239.

Fogo AB, Alpers CE, Cohen AH, Colvin RB, Jennette JC. Fundamentals of Renal Pathology. 2nd ed. 2014. Berlin, Heidelberg: Springer Berlin Heidelberg : Imprint: Springer; 2014.

Cianciosi D, Forbes-Hernández T, Afrin S, Gasparrini M, Reboredo-Rodriguez P, Manna P, et al. Phenolic compounds in honey and their associated health benefits: A review. Molecules. 2018;23(9).

Alvarez-Suarez J, Giampieri F, Battino M. Honey as a source of dietary antioxidants: structures, bioavailability and evidence of protective effects against human chronic diseases. CMC. 2013;20(5).

Herdhimas D. Pengaruh protektif pemberian madu personde terhadap gambaran histopatologi ginjal pada tikus wistar jantan yang diinduksi metanol. [Jawa Timur]: Universitas Jember; 2013.

Ndagu LF, Arjana AAG, Berata IK, Arjana. Madu berefek protektif terhadap infiltrasi sel radang dan perdarahan ginjal akibat induksi aspirin. Indonesia Med Veterinus. 2013;2(1).

Prasetyo A. Uji Protektif Madu pada Jaringan Duodenum Tikus Jantan Galur Wistar yang Diberi Pajanan Aspirin. Jurnal Kedokteran Unram; 2022; 11(2):833-837

Sogabe A, Uto H, Kanmura S, Nosaki T, Oyamada M, Tokunaga K, et al. Correlation of serum levels of complement C4a desArg with pathologically estimated severity of glomerular lesions and mesangial hypercellularity scores in patients with IgA nephropathy. International Journal of Molecular Medicine. 2013 Aug;32(2).

Roberts ISD, Troyanov S, Alpers CE, Amore A, Barratt J, Berthoux F, et al. The Oxford classification of IgA nephropathy: pathology definitions, correlations, and reproducibility. Kidney International. 2009 Sep;76(5).

Li Y, Wang J, Zhu X, Feng Q, Li X, Feng X. Urinary protein markers predict the severity of renal histological lesions in children with mesangial proliferative glomerulonephritis. BMC Nephrol. 2012 Dec;13(1).

Hosohata K. Role of oxidative stress in drug-induced kidney injury. IJMS. 2016;17(11).

Moneim AA, Ahmed OM, Fahim HI, Mohamed EE. The preventive effects of avocado fruit and seed extracts on cardio-nephrotoxicity induced by diethylnitrosamine/2-acetylaminoflurine in wistar rats. J Medicine. 2017;6(1).

Nelly. Efek protektif fraksi etil asetat daun kesum (Polygonum minus huds.) pada tikus putih galur wistar yang diinduksi cisplatin. Jurnal Untan. 2013;3(1).

Seely JC, Brix A. Kidney, Renal Tubule - Dilation. US Departement of Health and Human Services. 2014

Rita. Efek protektif fraksi metanol daun kesum (Polygonum minus huds.) pada tikus putih galur wistar yang diinduksi cisplatin. Jurnal Untan. 2013;3(1).

Rastogi V. Artefacts: A Diagnostic Dilemma – A Review. JCDR. 2013;

Roy C, Das S. The protective role of Aegle marmelos on aspirin-induced gastro-duodenal ulceration in albino rat model: A possible involvement of antioxidants. Saudi J Gastroenterol. 2012;18(3).

Raza H, John A. Implications of Altered Glutathione Metabolism in Aspirin-Induced Oxidative Stress and Mitochondrial Dysfunction in HepG2 Cells. Abdel-Naim AB, editor. PLoS ONE. 2012 Apr 30;7(4)

.

Mut-Salud N, Álvarez PJ, Garrido JM, Carrasco E, Aránega A, Rodríguez-Serrano F. Antioxidant intake and antitumor therapy: Toward nutritional recommendations for optimal results. Oxidative Medicine and Cellular Longevity. 2016;2016.

Rana AC, Gulliya B. Chemistry and pharmacology of flavonoids- A review. Indian Journal of Pharmaceutical Education and Research. 2019;53(1).

Yaman T, Yener Z, Celik I. Histopathological and biochemical investigations of protective role of honey in rats with experimental aflatoxicosis. BMC Complement Altern Med. 2016;16(1).

Banjarnahor SDS, Artanti N. Antioxidant properties of flavonoids. Med J Indones. 2014;23(4).

Oršolić N, Jazvinšćak Jembrek M, Terzić S. Honey and quercetin reduce ochratoxin A-induced DNA damage in the liver and the kidney through the modulation of intestinal microflora. Food and Agricultural Immunology. 2017 Sep 3;28(5):812–33.

Xu D, Hu M-J, Wang Y-Q, Cui Y-L. Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application. Molecules. 2019;24(6).

Lucida H, Agustin P, Suhatri S S. The Assay of Quercetin Solid Dispersion as a Potential Nephronprotector in Acute Renal Failure Induced Mice. PJ. 2019 Sep 7;11(5):907–12.

OO C, Ogundele OO, Akinmoladun AC, Famusiwa CD, Josiah SS, Olaleye MT, et al. Effects of catechin, quercetin and taxifolin on redox parameters and metabolites linked with renal health in rotenone-toxified rats. Niger J Physiol Sci. 2019;1(10).

Erboga M, Aktas C, Erboga ZF, Donmez YB, Gurel A. Quercetin ameliorates methotrexate-induced renal damage, apoptosis and oxidative stress in rats. Renal Failure. 2015 Oct 21;37(9).



Bookmark and Share
-->