The Relationship of Genetic Diversity of Human Immunodeficiency Virus-1 with Acquired Drug Resistance Mutation in HIV Patients on First-Lines Antiretroviral Therapy

Efrida Efrida✉, Andani Eka Putra, Ida Parwati, Jamsari Jamsari
DOI: https://doi.org/10.25077/mka.v45.i3.p400-407.2022

Abstract

Aim: This study aims to determine the relationship of HIV-1 genetic diversity with acquired drug resistance mutation in HIV-1 patients who receive first-line antiretroviral drugs at RSUP Dr. M. Djamil Padang. Methods: The study was conducted on 20 people with HIV who were diagnosed based on HIV-1 antibody screening tests using simple / rapid methods at the Central Laboratory of RSUP Dr. _ _ M. Djamil Padang and had received first-line ARV therapy for at least 6 months . Furthermore, proviral HIV-1 DNA extraction was carried out and amplification using a PCR (nested PCR technique) with a specific primer against reverse transcriptase at the Biomolecular Laboratory of the Microbiology Section of the Faculty of Medicine, Andalas University .  Result: The results showed that HIV-1 genetic diversity and changes in ARV regimens with the discovery of drug resistance mutations obtained for first-line ARVs were expected to be the basis for the management of HIV/AIDS patients. Conclusion: The conclusion of this study is that HIV-1 genetic diversity found is a form of recombinant CRF-AE and CRF- BC and subtypes A and B , a acquired drug resistance found is M184L and T215N (types of mutations that are resistant to NRTI- class ARVs) 

Keywords

Acquired Drug Resistance Mutation (ADRM), Antiretroviral TherapyHIV-1 , Genetic Diversity

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References

Alteri C, Svicher V, Gori C, D'Arrigo R, Ciccozzi M, Ceccherini-Silberstein F, et al , 2009. Karakterisasi pola mutasi resistansi obat pada pasien terinfeksi HIV-1 yang baru didiagnosis yang naif terhadap obat antiretroviral. Penyakit Menular BMC , 9: 111: 1-11.

Iyidogan P, Anderson KS, 2014. Perspektif saat ini tentang resistensi obat antiretroviral HIV-1. Virus , 6: 4095-4139.

Kotaki T, Khairunisa SQ, Witaningrum AM, Yunifiar MQ, Sukaartiningrum SD, Diansyah MN, et al ., 2015. HIV-1 menularkan mutasi resistansi obat di antara orang yang belum pernah ART di Surabaya, Indonesia. Penelitian dan Terapi AIDS ,12: 5:1-7.

Pyne MT, Hackett J, Holzmayer V, Hillyard DR, 2013. Analisis skala besar dari prevalensi dan distribusi geografis varian HIV-1 non-B di Amerika Serikat. JCM , 51(8): 2662-2669.

Kerina D, Babill SP, Muller F, 2013. Keragaman dan klasifikasi HIV, peran dalam penularan. Kemajuan dalam Penyakit Menular , 3: 146-156.

Gianella S, Richman DD, 2010. Varian minoritas HIV yang resistan terhadap obat. J Menginfeksi Dis , 202(5): 657-666.

Hosseinipour MC, Gupta RK, Zyl GV, Eron JJ, Nachega JB, 2013. Munculnya resistensi obat HIV selama terapi antiretroviral lini pertama dan kedua di rangkaian terbatas sumber daya. JID . 207(S2): S49-56.

Trotter AB, Hong SY, Srikantiah P, Abeyewickreme I, Bertagnolio S, Jordan MR, 2013. Tinjauan sistematis resistensi obat HIV di Wilayah Asia Tenggara Organisasi Kesehatan Dunia. AIDS Rev , 15(3): 162-170.

Vallabhaneni S, Chandy S, Heylen E, Ekstrand ML, 2013. Evaluasi kriteria imunologi WHO untuk kegagalan pengobatan: implikasi untuk deteksi kegagalan virologi, evolusi resistensi obat dan pilihan terapi lini kedua di India. JIAS, 16.1.18449.

Vidya M, Saravanan S, Uma S, Kumarasamy N, Sunil SS, Kantor R, et al., 2009. Resistensi obat HIV tipe-1 genotip di antara pasien dengan kegagalan imunologi terhadap terapi antiretroviral lini pertama di India Selatan. Antivir Ada , 14(7): doi: 10.3851/IMP1411.

Tang MW, Shafer RW, 2012. Resistensi antiretroviral HIV-1. Prinsip ilmiah dan aplikasi klinis. Narkoba , 72(9): e1-e25.

Li H, Zhong M, Guo W, Zhuang D, Li L, Liu Y, et al ., 2013. Prevalensi dan pola mutasi resistensi obat HIV dari 2010 hingga 2011 di antara individu yang gagal ART di Provinsi Yunnan, Cina. Plos SATU, 8(8): e 72630.

Rhee SY, Jordan MR, Raizes E, Chua A, Parkin N, Kantor R, et al , 2015. Mutasi resistensi obat HIV-1: aplikasi potensial untuk pengujian resistensi genotipik di tempat perawatan. PLoS ONE, 10(12): e0145772.

Roselinda daan jekti RP. 2012. Genotipe dan Penularan Human Immunodeficiency Virus-1 di tujuh provinsi di Indonesia. Ilmu Kesehatan Indonesia, 3(2):27-30

Efrida dan Eka Putra A. 2014. Sekuens terawetkan terkait HIV-1, IJCP dan ML, 20(2):133-140.



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