Efek Pemberian Induksi Etanol Absolut terhadap Perubahan Histologi Jaringan Gaster pada Tikus Model Gastritis

Hastuti Herman✉
DOI: https://doi.org/10.25077/mka.v45.i3.p367-373.2022

Abstract

Tujuan: Penelitian ini bertujuan untuk menilai pengaruh pemberian etanol absolut pada perubahan histologi dinding gaster. Metode: Tikus (Rattus norvegicus) jantan dengan berat badan 220g sebanyak delapan ekor dibagi kedalam dua kelompok (n = 4) yaitu kelompok kontrol tanpa pemberian etanol absolut dan kelompok gastritis yang diberi etanol absolut 1 ml. Setelah proses pembedahan dinding gaster dievaluasi secara makroskopik dan mikroskopik. Hasil: Induksi etanol absolut menyebabkan lesi hemoragik pada mukosa luminal gaster secara makroskopik. Sedangkan pengamatan histologi menunjukkan adanya tanda ulserasi, deskuamasi epitel, hemoragik akut pada mukosa, dan edema sub-mukosa, sedangkan muskularis tidak ditemukan kelainan pada lapisan muskularis dan serosa.  Kesimpulan: Etanol absolut yang diberikan pada tikus model gastritis menunjukkan lesi makroskopik berupa hemoragik mukosa luminal dan perubahan histologis berupa ulserasi, deskuamasi epitel, hemoragik akut, dan edema sub-mukosa, sedangkan lapisan muskularis dan serosa tampak normal.

Keywords

Gastritis; Etanol absolut; Gambaran histologi

Full Text:

PDF

References

Choi EY, Hwang HJ, Kim IH, Nam TJ. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats. Food Chem Toxicol [Internet]. 2009;47(1):134–9. Available from: http://dx.doi.org/10.1016/j.fct.2008.10.026

Shaker E, Mahmoud H, Mnaa S. Anti-inflammatory and anti-ulcer activity of the extract from Alhagi maurorum (camelthorn). Food Chem Toxicol [Internet]. 2010;48(10):2785–90. Available from: http://dx.doi.org/10.1016/j.fct.2010.07.007

Satyanarayana MN. Capsaicin and gastric ulcers. Crit Rev Food Sci Nutr. 2006;46(4):275–328.

Cologne. Gastritis: Overview. In: Institute for Quality and Efficiency in Health Care (IQWiG) [Internet]. 2018. Available from: https://www.ncbi.nlm.nih.gov/books/NBK310265/

Al Asmari A, Al Shahrani H, Al Masri N, Al Faraidi A, Elfaki I, Arshaduddin M. Vanillin abrogates ethanol induced gastric injury in rats via modulation of gastric secretion, oxidative stress and inflammation. Toxicol Reports [Internet]. 2016;3:105–13. Available from: http://dx.doi.org/10.1016/j.toxrep.2015.11.001

Choi YJ, Kim N, Lee JY, Nam RH, Seo JH, Lee S, et al. Gastroprotective effects of PMK-S005 against ethanol-induced acute gastric damage in rats. Gut Liver. 2016;10(3):348–55.

Yeo D, Hwang SJ, Kim WJ, Youn HJ, Lee HJ. The aqueous extract from Artemisia capillaris inhibits acute gastric mucosal injury by inhibition of ROS and NF-kB. Biomed Pharmacother [Internet]. 2018;99(September 2017):681–7. Available from: https://doi.org/10.1016/j.biopha.2018.01.118

Azer SA, Hossein Akhondi. Gastritis. In: In: StatPearls [Internet]. 2022. Available from: https://www.ncbi.nlm.nih.gov/books/NBK544250/

Lam JR, Schneider JL, Zhao W, Corley DA. Proton pump inhibitor and histamine 2 receptor antagonist use and vitamin B12 deficiency. JAMA - J Am Med Assoc. 2013;310(22):2435–42.

Singh A, Ashraf A. Hypercalcemic crisis induced by calcium carbonate. CKJ Clin Kidney J. 2012;5(4):288–91.

Freedberg DE, Kim LS, Yang YX. The Risks and Benefits of Long-term Use of Proton Pump Inhibitors: Expert Review and Best Practice Advice From the American Gastroenterological Association. Gastroenterology [Internet]. 2017;152(4):706–15. Available from: http://dx.doi.org/10.1053/j.gastro.2017.01.031

Jeon WY, Lee MY, Shin IS, Jin SE, Ha H. Curcuma aromatica water extract attenuates ethanol-induced gastritis via enhancement of antioxidant status. Evidence-based Complement Altern Med. 2015;2015:1–7.

Azab SS, Abdel Jaleelb GA, Eldahshanc OA. Anti-inflammatory and gastroprotective potential of leaf essential oil of Cinnamomum glanduliferum in ethanol-induced rat experimental gastritis. Pharm Biol. 2017;55(1):1654–61.

Kengkoom K, Tirawanchai NN, Angkhasirisap W, Ampawong S. Omeprazole preserves the RER in chief cells and enhances re-epithelialization of parietal cells with SOD and AQP-4 up-regulation in ethanol-induced gastritis rats. Exp Ther Med. 2017;14(6):5871–80.

Heidari F, Komeili-Movahhed T, Hamidizad Z, Moslehi A. The protective effects of rosmarinic acid on ethanol-induced gastritis in male rats: Antioxidant defense enhancement. Res Pharm Sci. 2021;16(3):305–14.

Boby N, Abbas MA, Lee EB, Im ZE, Hsu WH, Park SC. Protective effect of pyrus ussuriensis maxim. Extract against ethanol‐induced gastritis in rats. Antioxidants. 2021;10(3):1–17.

Lim JH, Kim JH, Kim N, Lee BH, Seo PJ, Kang JM, et al. Gastroprotective effect of cochinchina momordica seed extract in nonsteroidal anti-inflammatory drug-induced acute gastric damage in a rat model. Gut Liver. 2014;8(1):49–57.

Kim JY, Park S-D, Nam W, Nam B, Bae CH, Kim HJ, et al. R&BD Center, Korea Yakult Co., Ltd., Gyeonggi 17086, Korea. 2020;25(June):158–65.

Kopic S, Geibel JP. Gastric acid, calcium absorption, and their impact on bone health. Physiol Rev. 2013;93(1):189–268.

Kwon DA, Kim YS, Baek SH, Kim SK, Kim HK, Jo SK, et al. Protective effects of a standardized extract (HemoHIM) using indomethacin- and ethanol/HCl-induced gastric mucosal injury models. Pharm Biol [Internet]. 2019;57(1):543–9. Available from: https://doi.org/10.1080/13880209.2019.1651875

Yu L, Li R, Liu W, Zhou Y, Li Y, Qin Y, et al. Protective effects of wheat peptides against ethanol-induced gastric mucosal lesions in rats: Vasodilation and anti-inflammation. Nutrients. 2020;12(8):1–13.

Lee IC, Baek HS, Kim SH, Moon C, Park SH, Kim SH, et al. Effect of diallyl disulfide on acute gastric mucosal damage induced by alcohol in rats. Hum Exp Toxicol. 2015;34(3):227–39.



Bookmark and Share
-->